Developed in collaboration with 150 secondary school pupils and teachers for Stonewall, the posters feature the tagline in bold capital letters in white and black against a striking red background.
For the first time, the posters will appear on billboards for two weeks, from Monday 11 February 2008.
Ben Summerskill, Stonewall Chief Executive, says: "Homophobia is almost endemic in our schools and blights the lives of people throughout society. It makes sense that this zero-tolerance message should be extended to the wider public. Across urban and rural Britain, this plain-speaking slogan will remind people that discrimination against gay men and lesbians is no longer acceptable."
Stonewall, a gay lobbying group was founded in 1989 by a small group of women and men - including actor Sir Ian McKellen - who had been active in the struggle against Section 28 of the Local Government Act which was designed to prevent the so-called 'promotion' of homosexuality in schools.